Histone H3.3 Polyclonal Antibody/组蛋白H3.3多克隆抗体

货号：PA5-29602

规格：100 µL

价格：5334

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Recombinant protein fragment corresponding to a re

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1.74 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；2 µg/mL(ICC)；2 µg/mL(IF)；1:100-1:1000(IHC (P))；1:5,000(WB)

产品详细信息Recommended positive controls: Jurkat, Raji, K562, THP-1, HL-60, NCI-H929, NIH-3T3, JC, BCL-1, C2C12, Raw264.7, PC-12 , rice leaf.Predicted reactivity: Mouse (100%), Zebrafish (99%), Xenopus laevis (100%), Pig (100%), Rabbit (100%), Bovine (100%).Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a memberof the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

数据：

Histone H3.3 Antibody (PA5-29602) in IFImmunofluorescence analysis of Histone H3.3 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Histone H3.3 Rabbit Polyclonal Antibody (Product # PA5-29602) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. Histone H3.3 Antibody (PA5-29602) in WBWestern Blot analysis of Histone H3.3 was performed by separating 30 µg of various whole cell lysates by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a Histone H3.3 Polyclonal Antibody (Product # PA5-29602) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. A. NIH-3T3, B. JC, C. BCL-1, D. C2C12, E. Raw264.7.

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技术参数

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