H2BK5ac Polyclonal Antibody/H2BK5ac多克隆抗体

货号：PA5-17779

规格：100 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic acetylated peptide corresponding to resi

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	1.5 µg/1x10^6 cells(ChIP)；1:100(ICC)；1:100(IF)；1:100(IHC (P))；1:50(IP)；1:500(WB)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other acetylated histones.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

数据：

H2BK5ac Antibody (PA5-17779) in IFImmunofluorescence analysis of Acetyl-Histone H2B (Lys5) was performed using 70% confluent log phase HeLa cells treated with Trichostatin A. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H2B (Lys5) Rabbit Polyclonal Antibody (Product # PA5-17779) at 1:100 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased nuclear localization of Acetyl-Histone H2B (Lys5) upon Trichostatin A treatment. Panel e represents the untreated cells showing nuclear expression of Acetyl-Histone H2B (Lys5). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H2BK5ac Antibody (PA5-17779) in ChIPEnrichment of endogenous Acetyl-Histone H2B (Lys5) protein at specific gene loci using Anti-Acetyl-Histone H2B (Lys5) Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Acetyl-Histone H2B (Lys5) Rabbit Polyclonal Antibody (Product # PA5-17779, 3 µg) on sheared chromatin from 2 million Sodium butyrate-treated HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with relevant positive (ALDOA, GAPDH) and negative (MYOD, SAT2 satellite repeats) target genes/ binding sites.

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技术参数

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