SARS-CoV-2 Spike Protein S2 Polyclonal Antibody

货号：PA5-112048

规格：0.1mg

价格：4642

产品类型：抗体和染料

品牌：Thermo Fisher

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1 µg/mL(ELISA)；1 µg/mL(WB)

产品详细信息The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein.Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (100%)靶标信息It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

数据

SARS-CoV-2 Spike Protein S2 Antibody (PA5-112048) in WBWestern Blot analysis of SARS Coronavirus Spike Protein was performed in 293 cells. 15 µg of cell lysate was loaded per lane and the membrane was bloated with SARS-CoV-2 Spike Protein S2 Polyclonal Antibody (Product # PA5-112048) (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. A Goat anti-Rabbit IgG HRP conjugate at 1:10000 dilution was used to detect the primary antibody. Lane 1: WT 293 cells, Lane 2: Spike overexpressed 293 cells.

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