H3K9ac Monoclonal Antibody/H3K9ac单克隆抗体

货号：MA5-23517

规格：50 µg

价格：4532

产品类型：细胞分选

品牌：Alfa Aesar

类型:	单抗	同型对照：
浓度：	1 mg/mL	用法：	1:500(ICC)；1:500(IF)；1:3000(pep-ELISA)；1:1,000(WB)；1:2,000(Array)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9ac Antibody (MA5-23517) in IFImmunofluorescent detection of Acetyl-Histone H3 (Lys9) in HeLa cells stained with Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-23517). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9ac antibody (left) at a dilution of 1:500 in blocking solution followed by an anti-mouse antibody conjugated to AlexaFluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. H3K9ac Antibody (MA5-23517) in ICCImmunofluorescence analysis of Acetyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys9) Mouse Monoclonal Antibody (Product # MA5-23517) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents the untreated cells showing relatively lower expression of Acetyl-Histone H3 (Lys9). Panel f shows control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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