H3K9ac Monoclonal Antibody (J.924.2)/H3K9ac单克隆抗体（J.924.2）

货号：MA5-11195

规格：100 µL

价格：4692

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the amino termi

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：J.924.2

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1:200(Flow)；1:400(ICC)；1:400(IF)；1:800(IHC (P))；1:25(IP)；1:1000(WB)；

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other acetylated histones.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9ac Antibody (MA5-11195) in IFImmunofluorescent analysis of acetylated Histone H3 (Lys9, green) in HEK293T cells left untreated (top row) or treated with 5uM of the HDAC inhibitor, Scriptaid (bottom row), for 24 hours. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:50 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 at 40X magnification. Data courtesy of the Innovators Program. H3K9ac Antibody (MA5-11195) in WBImmunoprecipitation of acetylated Histone H3 (Lys9) was performed using whole cell lysates from HeLa cells left untreated (DMSO only) or cells treated with 0.3uM or 3 uM Trichostatin A (TSA) for 16 hours. Antigen-antibody complexes were formed by incubating 500 µg of the indicated lysate with 3 µg of an Acetyl Lysine monoclonal antibody (Product # MA1-2021) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µL Protein A/G Agarose (Product # 20421), washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer (Product # 39000). Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with an Acetyl-Histone H3 (Lys9) monoclonal antibody (Product # MA5-11195) at a dilution of 1:1000 overnight rotating at 4°C, washed in TBST, and probed with Clean-blot IP Detection Reagent (Product # 21230) at a dilution of 1:2000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34087).

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参考文献：

1. Frontiers in plant scienceCytomixis doesn't induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes.

技术参数

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