H3K9ac Recombinant Polyclonal Antibody (17HCLC)/H3K9ac重组多克隆抗体（17HCLC）

货号：710293

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetylated peptide corresponding to amino acids 5–13 of human Histone H3 [AcK9]

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：17HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-2 µg/mL(ICC)；1-2 µg/mL(IF)；1:1500(WB)；0.25 µg/mL(Array)

产品详细信息This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9ac Antibody (710293) in IFImmunofluorescent analysis of Acetyl-Histone Lys9 in HeLa cells treated with Sodium Butyrate (5mM for 24 hrs) using an Acetyl-Histone Lys9 Recombinant Rabbit Polyclonal Antibody (Product # 710293) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing nuclear localization of acetylated Histone H3 and Image E is a composite image of cells showing inhibition of antibody binding after competition with the acetylated peptide. H3K9ac Antibody (710293) in ARRAYThe specificity of the antibody, Anti-Acetyl-Histone H3 (Lys9) Recombinant Rabbit Polyclonal Antibody (Product # 710293, 0.25 µg/mL) to Histone H3K9Ac peptide was confirmed using MODified™ Histone Peptide Array (Active Motif, 13001). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used as the secondary antibody. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). The dot blot data obtained (lower panel) was analyzed using Array Analyze software as per the manufacturer's instructions (top panel).

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