H4ac pan-acetyl (K5,K8,K12) Polyclonal Antibody/H4ac泛乙酰（K5，K8，K12）多克隆抗体

货号：PA5-40083

规格：50 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH-conjugated synthetic peptide corresponding to

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.76 mg/mL	用法：	1.5 µg/1x10^6 cells(ChIP)；1:14,500(ELISA)；1:500(ICC)；1:500(IF)；1:1000(WB)；1:2,000(Array)；Assay dependant(ChIP-Seq)

靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H4ac pan-acetyl (K5,K8,K12) Antibody (PA5-40083) in IFImmunofluorescent detection of Acetyl-Histone H4 (Lys5 + Lys8 + Lys12) in HeLa cells using a Acetyl-Histone H4 (Lys5 + Lys8 + Lys12) polyclonal antibody (Product # PA5-40083). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. Figure 6A: cells were immunofluorescently labeled with the H4K5, 8, 12ac antibody (left) at a dilution of 1:500 in blocking solution followed by detection using an anti-rabbit antibody conjugated to AlexaFluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Figure 6B, C, D and E: staining of the cells with the H4K5, 8, 12ac antibody after incubation of the antibody with 10 ng/µl of the following blocking peptides: H4K5, 8, 12 unmodified (figure 6B), H4K5, 8, 12ac (figure 6C), H2A.ZK5, 7, 11ac (figure 6D) and H4K5, 8, 12, 16ac (figure 6E). H4ac pan-acetyl (K5,K8,K12) Antibody (PA5-40083) in ChIPChIP assays were performed on human K562 cells using a Acetyl-Histone H4 (Lys5 + Lys8 + Lys12) polyclonal antibody (Product # PA5-40083) and optimized PCR primer sets for qPCR. ChIP was performed on sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 µg per ChIP experiment was analyzed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

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