H4ac pan-acetyl (K5,K8,K12,K16) Polyclonal Antibody/H4ac泛乙酰（K5，K8，K12，K16）多克隆抗体

货号：PA5-40084

规格：50 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH-conjugated synthetic peptide corresponding to

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.76 mg/mL	用法：	1:21,200(ELISA)；1:250(ICC)；1:250(IF)；1:20,000(Array)

靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H4ac pan-acetyl (K5,K8,K12,K16) Antibody (PA5-40084) in ICCImmunofluorescence analysis of Acetyl-Histone H4 (Lys5, Lys8, Lys12, Lys16) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H4 (Lys5, Lys8, Lys12, Lys16) Rabbit Polyclonal Antibody (Product # PA5-40084) at 1:250 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows control cells with no primary antibody to assess background. The images were captured at 60X magnification. H4ac pan-acetyl (K5,K8,K12,K16) Antibody (PA5-40084) in ELISATo determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H4K5,8,12,16ac antibody (Product # PA5-40084) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:21,200.

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参考文献：

1. Frontiers in oncologyAspirin Inhibits Natural Killer/T-Cell Lymphoma by Modulation of VEGF Expression and Mitochondrial Function.

技术参数

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