SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Polyclonal Antibody

货号：PA5-81795

规格：100μl

价格：1698

产品类型：抗体和染料

品牌：Thermo Fisher

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	1 mg/mL	用法：	1:5000-1:10000(ELISA)1:1000-1:5000(WB)

产品详细信息PA5-81795 has cross-reactivity in ELISA and WB with SARS-CoV-2 (2019-nCoV) Spike S1 and SARS-CoV-2 (2019-nCoV) Spike RBD.PA5-81795 was prepared using the sequence encoding the S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667). This was expressed with a C-terminal polyhistidine tag.This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).This antibody has specificity for Human SARSCoronavirus Spike S1 Subunit Protein.靶标信息It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.

数据

SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) Antibody (PA5-81795) in WBWestern Blot analysis using a SARS Coronavirus Spike Protein (subunit 1) antibody (Product # PA5-81795). Proteins were loaded on a reducing SDS-PAGE. Lane A: SARS-CoV Spike S1, 5 ng; Lane B: SARS-CoV2 Spike S1, 30 ng; Lane C: SARS-CoV2 Spike RBD, 30 ng. Separated proteins were transferred to a membrane and probed with PA5-81795 at 1:2000 dilution. The membrane was then probed with goat anti-rabbit IgG (H+L), HRP conjugated secondary antibody diluted 1:10,000. The proteins were visualized using ECL.

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