H4K8ac Recombinant Polyclonal Antibody (9HCLC), ChIP-Verified/H4K8ac重组多克隆抗体（9HCLC），ChIP认证

货号：710828

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Acetylated peptide (Lys8) corresponding to human H

物种：人

宿主：兔

抗体亚型：IgG

克隆号：9HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	1-5 µg(ChIP)；1 µg/mL(ICC)；1 µg/mL(IF)；0.5-1 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producinghigher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H4K8ac Antibody (710828) in IFImmunofluorescence was performed on fixed and permeabilized A549 cells for detection of Histone H4K8ac using Anti- Histone H4K8ac Recombinant Rabbit Polyclonal Antibody (Product # 710828, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of Histone H4K8ac protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H4K8Ac. Panel e) represents control cells with no primary Antibody to assess background. H4K8ac Antibody (710828) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of A431 (Lane1), A431 treated with 0.5 uM SAHA for 24 hours (Lane 2), HeLa (Lane 3), HeLa treated with 0.5 uM SAHA for 24 hours (Lane 4), HeL 92.1.7 treated with 0.5 uM SAHA for 24 hours (Lane 5), HT 29 treated with 0.5 uM SAHA for 24 hours (Lane 6), HeLa treated with 5 mM sodium butyrate (Lane 7). The blots were probed with Anti-Histone H4K8ac Recombinant Rabbit Polyclonal Antibody (Product # 710828, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 17kDa band corresponding to Histone H4K8ac was observed across cell lines according to the given treatment. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% nonfat milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

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