H3K17me2aK18ac Polyclonal Antibody/H3K17me2aK18ac多克隆抗体

货号：PA5-40094

规格：50 µg

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：KLH-conjugated synthetic peptide corresponding to

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.94 mg/mL	用法：	1 µg(ChIP)；1:28,800(ELISA)；1:500(ICC)；1:500(IF)；1:1000(WB)；1:20,000(Array)；Assay dependant(ChIP-Seq)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K17me2aK18ac Antibody (PA5-40094) in IFImmunofluorescent detection of Di-Methy-Acetyl-Histone H3 (Arg17 + Lys18) in NIH3T3 cells using a Di-Methy-Acetyl-Histone H3 (Arg17 + Lys18) polyclonal antibody (Product # PA5-40094). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3R17me2 (asym)K18ac antibody (left) at a dilution of 1:500 in blocking solution followed by detection using an anti-rabbit antibody conjugated to AlexaFluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. H3K17me2aK18ac Antibody (PA5-40094) in ChIPChIP assays were performed on human HeLa cells using a Di-Methy-Acetyl-Histone H3 (Arg17 + Lys18) polyclonal antibody (Product # PA5-40094) and optimized PCR primer pairs for qPCR. ChIP was performed with sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

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