CD282 (TLR2) Monoclonal Antibody (TL2.1), Functional Grade, eBioscience™/CD282 (TLR2) 单克隆抗体(TL2.1), 功能抗体

货号：16-9922-82,16-9922-38

规格：100 µg,5 mg

价格：3154,23374

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

荧光染料：其它

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.Applications Reported: TL2.1 has been reported for use in flow cytometric analysis and blocking of TLR2-mediated cytokine production. Inhibition of cytokine production has been reported in the literature using Listeria monocytogenes and peptidoglycans. Optimal conditions for the inhibition assays using TL2.1 and specific stimulatory reagents/suppliers have to be evaluated by individual investigators. Neutralization Protocol: (Important note: make sure to eliminate LPS contamination in the culture system) 1) Prepare target cells of interest at the concentration of 2 x 10^6 /mL in 10% FBS-IMDM (or 5% human serum). 2) Pre-incubate the cells with 10-20 µg/mL of TL2.1 or isotype control antibody at room temperature for 30min. 3) Add the stimuli and incubate at 37°C, 5% CO2 for 14 hours. Harvest the supernatants. 4) Follow the protocol of eBioscience Ready-SET-Go ELISA set to detect IL-6 or TNF alpha production.Applications Tested: The TL2.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD282 (TLR2) Antibody (16-9922-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD282 (TLR2) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

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参考文献：

1.PloS oneHIV-1 Tat Protein Induces Production of Proinflammatory Cytokines by Human Dendritic Cells and Monocytes/Macrophages through Engagement of TLR4-MD2-CD14 Complex and Activation of NF-κB Pathway."16-9922 was used in Inhibition experiments to reveal a novel mechanism by which HIV-1 hijacks the TLR4 pathway, establishing abnormal hyper-activation of the immune system."AuthorsBen Haij N,Planès R,Leghmari K,Serrero M,Delobel P,Izopet J,BenMohamed L,Bahraoui E2.Scientific reportsDifferential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release."16-9922 was used in Blocking experiments to explore neutrophil responses, in particular the release of sepsis-associated factors heparin-binding protein and resistin, in relation to specific bacterial stimuli and sepsis of varying aetiology."AuthorsSnäll J,Linnér A,Uhlmann J,Siemens N,Ibold H,Janos M,Linder A,Kreikemeyer B,Herwald H,Johansson L,Norrby-Teglund A3.PloS oneEnterovirus-71 virus-like particles induce the activation and maturation of human monocyte-derived dendritic cells through TLR4 signaling."16-9922 was used in Neutralization experiments to elucidate the mechanisms through which enterovirus 71 virus-like particles induce cell-mediated immunity."AuthorsLin YL,Hu YC,Liang CC,Lin SY,Liang YC,Yuan HP,Chiang BL4.Infection and immunityBystander macrophage apoptosis after Mycobacterium tuberculosis H37Ra infection."16-9922 was used in Blocking experiments to investigate bystander apoptosis of macrophages not infected with M.tuberculosis."AuthorsKelly DM,ten Bokum AM,O'Leary SM,O'Sullivan MP,Keane J5.The Journal of investigative dermatologyDistinct strains of Propionibacterium acnes induce selective human beta-defensin-2 and interleukin-8 expression in human keratinocytes through toll-like receptors."16-9922 was used in Blocking experiments to suggest that Propionibacterium acnes modulates the antimicrobial peptide and chemokine expression of keratinocytes."AuthorsNagy I,Pivarcsi A,Koreck A,Széll M,Urbán E,Kemény L

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