H3K36me2 Monoclonal Antibody (T.571.7), ChIP-Verified/H3K36me2单克隆抗体（T.571.7），ChIP认证

货号：MA5-14867

规格：100 µL

价格：1

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the amino termi

物种：其它

宿主：兔

抗体亚型：IgG

克隆号：T.571.7

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1-5 µg(ChIP)；1:100(ICC)；1:100(IF)；1:25(IHC (P))；1 µg/mL(WB)；0.25 µg/mL(Array)

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with di-methylated histone H3 Lys4, Lys9, Lys27, Lys79 or methylated histone H4 Lys20.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K36me2 Antibody (MA5-14867) in IFImmunofluorescent analysis of Di-Methyl-Histone H3 (Lys36) in HeLa cells using a Di-Methyl-Histone H3 (Lys36) monoclonal antibody (Product # MA5-14867) (green). Actin filaments are labeled with a fluorescent red phalloidin. H3K36me2 Antibody (MA5-14867) in ChIPK-MetStat Panel, SNAP-ChIP™ Spike-in (Product # A47356), a proprietary technology developed byEpiCypher™was used to analyze the performance of H3K36me2 antibody (Product # MA5-14867) in ChIP. SNAP-ChIP panels consist of a pool of DNA-barcoded recombinant nucleosomes harboring histone PTMs that are spiked-in to a ChIP reaction to assess efficiency and specificity of the antibody. The K-MetStat panel includes an unmodified control plus nucleosomes containing histones with mono, di and tri-methyl forms of lysine residues on histone H3 and H4 as shown on the x-axis. Recovery of each unique DNA-barcoded nucleosome is quantified to determine how much of each PTM is immunoprecipitated in the ChIP reaction (for more information seereference). H3K36me2 antibody was tested in native ChIP with 3 µg K-562 cell chromatin and 3 µg antibody. Specificity (left Y-axis) was determined by qPCR to each modified nucleosome in the panel (X-axis). Black bar represents antibody efficiency (right Y-axis; log scale) and indicates percentage of the barcoded nucleosome target immunoprecipitated relative to Input. All bars represent mean ± SEM.

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