H3K36me2 Recombinant Polyclonal Antibody (8HCLC)/H3K36me2重组多克隆抗体（8HCLC）

货号：710799

规格：100 µg

价格：4692

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Peptide corresponding to Human HIST1H3A (aa 33-41)

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

克隆号：8HCLC

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.156-10 µg/mL(ELISA)；1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with Rat, Cat, Pig and Monkey.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonalantibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K36me2 Antibody (710799) in WBWestern blot analysis was performed on acid extracts (30 µg lysate) of HCT116 (Lane1), HeLa (Lane2), A-375 (Lane3) and K-562 (Lane4). The blots were probed with Anti-H3K36me2 Recombinant Rabbit Polyclonal Antibody (Product # 710799, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 17 kDa band corresponding to H3K36me2 was observed across cell line tested. To confirm the specificity of Anti-H3K36me2 Recombinant Rabbit Monoclonal Antibody, competition was performed with the phosphopeptide (10 µg/mL) as shown in the corresponding blot on the right. The peptide competes with the antibody and prevents it from binding to the target protein. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). H3K36me2 Antibody (710799) in ELISACross-reactivity ELISA for Anti-Histone H3K36me2 Recombinant Rabbit Polyclonal Antibody (Product # 710799) was performed against H3K36me2, H3K36me1, H3K36 Nme and H3K36me3 by coating respective antigens at 0.25 µg/mL. Antibodies at 10, 2.5, 0.625 and 0.156 µg/mL were added and detected using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). The plate was developed using Stabilized Chromogen, TMB (Product # SB02).

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