H3K4me2 Polyclonal Antibody/H3K4me2多克隆抗体

货号：PA5-31912

规格：100 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to a region within

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.52 mg/mL	用法：	1:100-1:1,000(ICC)；1:100-1:1,000(IF)；1:100-1:1,000(IHC (P))；1:500-1:1000(IP)；1:1,000-1:10,000(WB)

产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K4me2 Antibody (PA5-31912) in IFImmunofluorescence analysis of Di-Methyl-Histone H3 (Lys4)was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # PA5-31912) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K4me2 Antibody (PA5-31912) in ICCImmunocytochemistry-Immunofluorescence analysis of Di-Methyl-Histone-H3-(Lys4) was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Di-Methyl-Histone H3 (Lys4) Polyclonal Antibody (Product # PA5-31912) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-46471.htm