H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified

货号：MA5-11198

规格：100 µL

价格：5930

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the amino termi

物种：大鼠

宿主：兔

抗体亚型：IgG

克隆号：G.299.10

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1-5 µg (ChIP)；1:200(IHC(P))；1:1600(ICC)；1:1600(IF)；1:1,000(WB)；1:2,000(Array)

靶标信息H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

数据：

H3K27me3 Antibody (MA5-11198) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys27) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Monoclonal Antibody (Product # MA5-11198) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K27me3 Antibody (MA5-11198) in WB Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), NIH/3T3 (Lane 2), HCT 116 (Lane 3), C2C12 (Lane 4), Hep G2 (Lane 5) and MCF7 (Lane 6). The blot was probed with Anti-Tri-Methyl-Histone H3 (Lys27) (Product # MA5-11198, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Tri-Methyl-Histone H3 (Lys27) was observed across the cell lines tested.

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