Histone H2A Recombinant Rabbit Monoclonal Antibody (15H4L7)

货号：700158

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A peptide corresponding to amino acids 2-19 of hum

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：15H4L7

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	0.1-2 µg/mL(WB)

产品详细信息This antibody is predicted to react with mouse based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

Histone H2A Antibody (700158) in WB Western blot analysis of Histone 2A in HeLa (lane 1) NIH-3T3 (lane 2) and COS7 cell lysate (lane 3) (30 µg/each lane) using a Histone 2A recombinant rabbit monoclonal antibody (Product # 700158) at a dilution of 1 µg/mL. Samples were blocked in 5% milk/TBST and detection was performed using an AP-conjugated goat anti-rabbit secondary antibody at a dilution of 1:10,000. NBT/BCIP was used as the substrate (Product # WB7105).

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