H3K27me3 Polyclonal Antibody/H3K27me3多克隆抗体

货号：PA5-31817

规格：100 µL

价格：5547

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to a region within

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.28 mg/mL	用法：	Assay Dependent(DB)；1:100-1:1000(ICC)；1:100-1:1000(IF)；1:100-1:1000(IHC (F))；1:100-1:500(IHC (Free))；1:100-1:1000(IHC (P))；Assay Dependent(IM)；1:500-1:3,000(WB)；1:2,000(Array)

产品详细信息Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, K562, THP-1, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.靶标信息H3K27me3 is the epigenetic mark where histone H3 is trimethylated at lysine residue 27. H3K27me3 is an epigenetic silencing mark and thus associated with downregulation of nearby genes and often found in heterochromatin regions. H3K27me3 is mainly found at inactive gene promoters and generally is found in opposition to the activating mark H3K4me3. H3K27me3 often occupies gene rich regions with close association to development regulators. EZH2, a component of the PRC2 complex, is the histone methyltransferase (or writer) responsible for trimethylating H3K27. UTX and JMJD3 both are histone demethylases (or erasers) for H3K27me3. The regulation of H3K27me3 is crucial for gene regulation and dysregulation of H3K27me3 has been implicated in numerous diseases including cancer. H3K27me3 antibodies are often used for western blot and ChIP applications to study changes in levels and occupancy of H3K27me3.

数据：

H3K27me3 Antibody (PA5-31817) in IFImmunofluorescent analysis of Tri-Methyl-Histone H3 mLys27 in paraformaldehyde-fixed A431 cells using a Tri-Methyl-Histone H3 mLys27 polyclonal antibody (Product # PA5-31817) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled withProduct # PA5-29281(Red) at a 1:2000. H3K27me3 Antibody (PA5-31817) in IHC (F)Immunohistochemistry (Frozen) analysis of Tri-Methyl-Histone-H3-(Lys27) was performed in frozen sections of mouse liver tissue. Red: Tri-Methyl-Histone H3 (Lys27) Polyclonal Antibody (Product # PA5-31817) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody. Blue: Nuclear staining with Hoechst 33342. Antigen Retrieval: EDTA based buffer, pH 8.0 buffer, 15min.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. Scientific reportsLong non-coding RNA PARTICLE bridges histone and DNA methylation.2. Scientific reportsLong non-coding RNA PARTICLE bridges histone and DNA methylation.

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-46491.htm