H3K9me3 Polyclonal Antibody/H3K9me3多克隆抗体

货号：49-1008

规格：50 µg

价格：5335

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：rabbits against histone H3, trimethylated at lysin

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：	0.9 mg/mL	用法：	1:20,000(DB)；1:100-1:1000(ELISA)；1:1000(ICC)；1:1000(IF)；1:1,000(WB)；0.25 µg/mL(Array)

靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail ofhistone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

数据：

H3K9me3 Antibody (49-1008) in IFImmunofluorescence analysis of Tri-Methyl-Histone H3 (Lys9) was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rabbit Polyclonal Antibody (Product # 49-1008) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. H3K9me3 Antibody (49-1008) in ELISATo determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me3 antibody (Product # 49-1008) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.

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参考文献：

1. The Journal of cell biologySilencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia.2. Experimental cell researchDNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop.3. GeneEpigenetic drift towards histone modifications regulates CAV1 gene expression in colon cancer.4. Nucleic acids researchUpregulating endogenous genes by an RNA-programmable artificial transactivator.5. Oncology reportsLong non-coding RNA urothelial carcinoma associated1 induces cell replication by inhibiting BRG1 in 5637 cells.

技术参数

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