H4K20me3 Recombinant Rabbit Monoclonal Antibody (12 H2L19)/H4K20me3重组兔单克隆抗体（12 H2L19）

货号：701777

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Methylated peptide (Lys20) corresponding to Human

物种：人

宿主：兔

抗体亚型：IgG

克隆号：12 H2L19

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µg/mL(ICC)；1 µg/mL(IF)；1:1500(WB)；0.25 µg/mL(Array)

产品详细信息701777 detects H4K20me3 in human and drosophila cells in WB, flow, ICC, IF and ChIP applications.Since it is highly conserved across species, the antibody may react with many other species.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

数据：

H4K20me3 Antibody (701777) in IFImmunofluorescence was performed on fixed and permeabilized HeLa cells for detection of Histone H4K20me3 using Anti-Histone H4K20me3 Recombinant Rabbit Monoclonal Antibody (Product # 701777, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Histone H4K20me3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Histone H4K20me3. Panel e) represents control cells with no primary Antibody to assess background. H4K20me3 Antibody (701777) in ARRAYThe specificity of the antibody, Anti-Tri-Methyl-Histone H4 (Lys20) Recombinant Rabbit Monoclonal Antibody (Product # 701777, 0.25 µg/mL) to Histone H4K20me3 peptide was confirmed using MODified™ Histone Peptide Array (Active Motif, 13001). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) was used as the secondary antibody. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106). The dot blot data obtained (lower panel) was analyzed using Array Analyze software as per the manufacturer's instructions (top panel).

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