Phospho-ETS2 (Thr72) Polyclonal Antibody/Phospho-ETS2（Thr72）多克隆抗体

货号：44-1105G

规格：100 µL

价格：5164

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：The antiserum was produced against a chemically sy

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	1-5 µg(ChIP)；1:250(ICC)；1:250(IF)；1:10-1:50(IHC (P))；1:1000(WB)

靶标信息ETS transcriptions factors, such as ETS2, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes.

数据：

Phospho-ETS2 (Thr72) Antibody (44-1105G) in IFImmunofluorescence analysis of Phospho-ETS2 pThr72 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-ETS2 pThr72 Rabbit Polyclonal Antibody (Product # 44-1105G)at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. Phospho-ETS2 (Thr72) Antibody (44-1105G)The EMBO journal 2012 -Published figure using Phospho-ETS2 (Thr72) polyclonal antibody (Product # 44-1105G)

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参考文献：

1. CellStem Cell Lineage Infidelity Drives Wound Repair and Cancer.2. eLifeETS family transcriptional regulators drive chromatin dynamics and malignancy in squamous cell carcinomas.3. FEBS lettersRuvBl2 cooperates with Ets2 to transcriptionally regulate hTERT in colon cancer.4. eLifeETS family transcriptional regulators drive chromatin dynamics and malignancy in squamous cell carcinomas.5. The EMBO journalInactivation of Rb in stromal fibroblasts promotes epithelial cell invasion.

技术参数

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