Phospho-JNK1/JNK2 (Thr183, Tyr185) Recombinant Rabbit Monoclonal Antibody (D12H7L17)/Phospho-JNK1 / JNK2（Thr183，Tyr185）重组兔单克隆抗体（D12H7L17）

货号：700031

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A peptide corresponding to amino acids 179-189 and

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：D12H7L17

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µL(ChIP)；1-5 µg/mL(ELISA)；0.5-5 µg/1x10^6 cells(Flow)；4-6 µg/mL(ICC)；4-6 µg/mL(IF)；1:10-1:100(IHC (P))；0.1-1 µg/mL(WB)

产品详细信息This antibody is predicted to react with mouse, rat, primate, canine, bovine, porcine, chicken, equine, zebrafish and Xenopus based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

数据：

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (700031) in IFImmunofluorescent analysis of Phospho-JNK1/JNK2 pThr183+pTyr185 in HeLa cells using a Phospho-JNK1/JNK2 pThr183+pTyr185 recombinant rabbit monoclonal antibody (Product # 700031) at a dilution of 5 µg/mL in the absence of peptide (left) or in the presence of the immunogenic peptide (right), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (700031) in IHC (P)Immunohistochemistry analysis of JNK1/2 (pT183/pY185) showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with JNK1/2 (pT183/pY185) Monoclonal antibody (Product # 700031) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using a HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

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参考文献：

1. PloS oneTicagrelor induces paraoxonase-1 (PON1) and better protects hypercholesterolemic mice against atherosclerosis compared to clopidogrel.2. Cancer researchEnhanced Glycolysis Supports Cell Survival in EGFR-Mutant Lung Adenocarcinoma by Inhibiting Autophagy-Mediated EGFR Degradation.3. Scientific reportsGDF15 deficiency exacerbates chronic alcohol- and carbon tetrachloride-induced liver injury.4. Toxicology researchNanoparticulate titanium dioxide-inhibited dendritic development is involved in apoptosis and autophagy of hippocampal neurons in offspring mice.5. The European journal of neuroscienceTumor necrosis factor-alpha mediates activation of NF-κB and JNK signaling cascades in retinal ganglion cells and astrocytes in opposite ways.

技术参数

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