Phospho-JNK1/JNK2 (Thr183, Tyr185) Monoclonal Antibody (E.665.10)/Phospho-JNK1 / JNK2（Thr183，Tyr185）单克隆抗体（E.665.10）

货号：MA5-15228

规格：200 µL

价格：4880

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic phosphopeptide corresponding to residues

物种：其它

宿主：小鼠

抗体亚型：其它

克隆号：E.665.10

荧光染料：其它

类型:	单抗	同型对照：
浓度：		用法：	1-3 µL(ChIP)；1:400(Flow)；1:200(ICC)；1:200(IF)；1:250(IP)；1:2000(WB)

产品详细信息Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.It is not recommended to aliquot this antibody.靶标信息C-Jun N-terminal Kinase (JNK) is also known as Mitogen-activated protein kinase (MAPK8). It belongs to the MAPK superfamily of stress-activated protein kinases. MAPKs are Serine-threonine protein kinases that are activated in response to a variety of extracellular stimuli, and mediate signal transduction from the cell surface to the nucleus. JNK is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. JNK pathways are activated by stress and inflammatory signals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2.

数据：

Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (MA5-15228) in IFImmunofluorescent analysis of Phospho-JNK (green) in HeLa cells either left untreated (left panel) or treated with 25 µg/mL anisomycin (right panel) for 25 min. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-15228) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification. Phospho-JNK1/JNK2 (Thr183, Tyr185) Antibody (MA5-15228) in FlowFlow cytometric analysis of Phospho-JNK on Jurkat cells left untreated (green histogram) or treated with anisomycin (blue histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-SAPK/JNK pThr183/Tyr185 monoclonal antibody (Product # MA5-15228) at a dilution of 1:200 for 1 hour at room temperature. As a negative control anisomycin-treated cells were stained with an isotype control (red histogram). Following staining with either MA5-15228 or isotype control, cells were stained with a fluorescently-conjugated anti-mouse IgG secondary antibody and analyzed on a flow cytometer.

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参考文献：

1. Cancer researchSyndecan-1 (CD138) Suppresses Apoptosis in Multiple Myeloma by Activating IGF1 Receptor: Prevention by SynstatinIGF1R Inhibits Tumor Growth.2. Journal of immunology (Baltimore, Md. : 1950)Duox1-derived H2O2 modulates Cxcl8 expression and neutrophil recruitment via JNK/c-JUN/AP-1 signaling and chromatin modifications.

技术参数

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