Phospho-p53 (Ser15) Recombinant Rabbit Monoclonal Antibody (14H61L24)/Phospho-p53（Ser15）重组兔单克隆抗体（14H61L24）

货号：700439

规格：100 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：A peptide corresponding to amino acids 9-19 of P04

物种：人

宿主：兔

抗体亚型：IgG

克隆号：14H61L24

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1 µL(ChIP)；0.5-5 µg/1x10^6 cells(Flow)；Assay-dependent(FRET)；0.5-2 µg/mL(ICC)；0.5-2 µg/mL(IF)；0.1-0.5 µg/mL(WB)

产品详细信息This antibody is predicted to react with chimpanzee, guinea pig, porcine, ovine, bovine, equine, feline, Rhesus monkey and Xenopus based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

数据：

Phospho-p53 (Ser15) Antibody (700439) in IFImmunofluorescent analysis of p53 (pS15) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with p53 (pS15) Recombinant Rabbit Monoclonal Antibody (Product # 700439) at 2 µg/mL and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e shows no primary antibody control. The images were captured at 20X magnification. Phospho-p53 (Ser15) Antibody (700439) in IFImmunofluorescent analysis of Phospho-p53 pSer15 in U2OS cells treated with 0.25 µg/mL Camptothecin (right) and untreated cells (left) using a Phospho-p53 pSer15 recombinant rabbit monoclonal antibody (Product # 700439) at a dilution of 1 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000.

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参考文献：

1. Experimental cell researchProtective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes.2. Toxicological sciences : an official journal of the Society of ToxicologyProfiling dose-dependent activation of p53-mediated signaling pathways by chemicals with distinct mechanisms of DNA damage.3. Toxicological sciences : an official journal of the Society of ToxicologyProfiling dose-dependent activation of p53-mediated signaling pathways by chemicals with distinct mechanisms of DNA damage.3. Journal of the American College of SurgeonsAutomated quantitative analysis of tissue microarray of 443 patients with colorectal adenocarcinoma: low expression of Bcl-2 predicts poor survival.

技术参数

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