Phospho-STAT1 (Tyr701) Monoclonal Antibody (ST1P-11A5)/磷酸化STAT1（Tyr701）单克隆抗体（ST1P-11A5）

货号：33-3400

规格：50 µg

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic phospho peptide encompassing the conserv

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：ST1P-11A5

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	5 µg(ChIP)；0.1-1.0 µg/mL(ELISA)；1:10-1:100(IHC (P))；0.5-2 µg/mL(WB)；(ICC)；(IF)；(IF)

产品详细信息This antibody reacts specifically with the tyrosine-701 phosphorylated form of STAT1 and does not exhibit appreciable cross-reactivity with corresponding tyrosine phosphorylated forms of other STAT proteins or with other endogenous phosphotyrosine-containing proteins. To confirm the exclusive recognition of tyrosine phosphorylated STAT1, Western blots were carried out on lysates of 293 cells transfected with a STAT1 expression vector together with a wild type or kinase “dead” JAK1 expression vector. In addition, recognition of endogenous tyrosine phosphorylated STAT1 was confirmed on Western blots with cell lysates derived from serum starved or EGF-stimulated A431 cells and on Western blots with IFNa-stimulated mouse embryo fibroblast lysates.靶标信息STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.

数据：

Phospho-STAT1 (Tyr701) Antibody (33-3400) in IHC (P)Immunohistochemistry analysis of Phospho-STAT1 (pTyr701) showing staining in the nucleus and cytoplasm of paraffin-embedded human cervical carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Phospho-STAT1 pTyr701monoclonal antibody (Product # 33-3400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Phospho-STAT1 (Tyr701) Antibody (33-3400) in WBWestern blot analysis of STAT1 (pT701) was performed by loading 20 µg of NIH/3T3 (lane1), NIH/3T3 treated for 10 minutes with 50 ng/mL of PDGF (lane2), HeLa (lane3), HeLa treated for 30 minutes with 100 ng/mL of IFN alpha (lane4), HeLa treated for 30 minutes with 100 ng/mL of IFN gamma (lane5), HeLa treated for 10 minutes with 50 ng/mL of PDGF (lane6) and Karpas 299 (lane7) cell lysate using Novex®NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. STAT1 (pT701) was detected at ~ 90 kDa using STAT1 (pT701) Mouse Monoclonal Antibody (Product # 33-3400) at 0.5-1 µg/mL in 5% skim milk for 3 hour at room temperature on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. Molecular visionAnalysis of interleukin-20 receptor complexes in trabecular meshwork cells and effects of cytokine signaling in anterior segment perfusion culture.2. Scientific reportsTWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice.3. Scientific reportsTWEAK blockade decreases atherosclerotic lesion size and progression through suppression of STAT1 signaling in diabetic mice.4. PLoS pathogensVaccinia Virus Protein C6 Inhibits Type I IFN Signalling in the Nucleus and Binds to the Transactivation Domain of STAT2.5. PLoS pathogensVaccinia Virus Protein C6 Inhibits Type I IFN Signalling in the Nucleus and Binds to the Transactivation Domain of STAT2.

技术参数

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