Phospho-STAT3 (Ser727) Polyclonal Antibody/磷酸化STAT3（Ser727）多克隆抗体

货号：44-384G

规格：100 µL

价格：4809

产品类型：抗体和染料

品牌：Thermo Fisher

抗原：Synthetic phosphopeptide from human STAT3 containi

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	多抗	同型对照：
浓度：		用法：	10 µL(ChIP)；1:10-1:100(IHC (P))；(IF)；1:1000(WB)；(IHC)；(IP)

靶标信息STAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the nucleus, and act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for the transcriptional activation by STAT3. STAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but not IFN-beta. The transcription factors associated with STAT3 are c-Jun and cyclic AMP-responsive enhancer binding protein (CREB). STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3 while the PIAS3 protein is a specific inhibitor of STAT3. Three alternatively spliced transcript variants encoding distinct isoforms of STAT3 have been described. Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage.

数据：

Phospho-STAT3 (Ser727) Antibody (44-384G) in IHC (P)Immunohistochemistry analysis of STAT3 [pS727] showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a STAT3 [pS727] Rabbit Polyclonal Antibody (44384G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. Phospho-STAT3 (Ser727) Antibody (44-384G) in WBWestern blot analysis of STAT3 [pS727] was performed by loading 20 µg of NIH/3T3 (lane1), NIH/3T3 treated for 10 minutes with 50 ng/mL of PDGF (lane2), NIH/3T3 treated for 10 minutes with 200 ng/mL of EGF (lane3), A549 (lane4) and A549 treated for 10 minutes with 200 ng/mL of EGF (lane5) cell lysate using Novex®NuPAGE® 4-12 % Bis-Tris gel (NP0322BOX), XCell SureLock Electrophoresis System (EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. STAT3 [pS727] was detected at ~83 kDa using STAT3 [pS727] Rabbit Polyclonal Antibody (44384G) at 1:1000 dilution in 5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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参考文献：

1. Molecular medicine reportsATM‑JAK‑PD‑L1 signaling pathway inhibition decreases EMT and metastasis of androgen‑independent prostate cancer.2. The Journal of experimental medicineGuanylate binding protein 1 is a novel effector of EGFR-driven invasion in glioblastoma.3. Journal of molecular neuroscience : MNThe Cdk5/p35 kinases modulate leptin-induced STAT3 signaling.4. The Journal of experimental medicineGuanylate binding protein 1 is a novel effector of EGFR-driven invasion in glioblastoma.5. PloS oneEarly-onset and robust amyloid pathology in a new homozygous mouse model of Alzheimer's disease.

技术参数

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