Phospho-PKC theta (Thr538) Recombinant Rabbit Monoclonal Antibody (F4H4L1)

货号：700043

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：A peptide corresponding to amino acids 531-539 of

物种：人

宿主：兔

抗体亚型：IgG

克隆号：F4H4L1

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:100-1:500(Flow) ;4-6 µg/mL(IHC(P));2-4 µg/mL(WB)

产品详细信息This antibody is predicted to react with bovine, chimpanzee, mouse, rat and Xenopus based on sequence homology.Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. 靶标信息Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.

数据：

Phospho-PKC theta (Thr538) Antibody (700043) in IHC (P) Immunohistochemistry analysis of Phospho-PKC theta pThr538 in formalin-fixed, paraffin-embedded human breast carcimona tissue using a Phospho-PKC theta pThr538 monoclonal antibody (Product # 700043) at a dilution of 5 µg/mL. Staining was visualized using DAB. Images were taken at a magnification of 20x. Results show cytoplasmic staining in tumor cells. Phospho-PKC theta (Thr538) Antibody (700043) in WBWestern blot analysis of PKC-theta (pT538) was performed by loading 30 µg of HEK-293 (lane1) HEK-293 treated for 20 minutes with 200 nM of PMA (lane2), Jurkat (lane3) and Jurkat treated for 20 minutes with 200 nM of PMA (lane4) cell lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® 2 Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. PKC-theta (pT538) was detected at ~80 kDa using PKC-theta (pT538) Rabbit Monoclonal Antibody (Product # 700043) at 1-2 µg/mL in 5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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