ATM Monoclonal Antibody (2C1)

货号：MA1-23152

规格：100 µL

价格：5853

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant protein expressed in E. coli correspon

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：2C1

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	5 ug/ml(IHC(P));1-10 ug/ml(IP)；1:500-1:3000（WB）

产品详细信息Recommended positive controls: SK-N-SH, HeLa, HeLa nuclear extract.Predicted molecular weight of MA1-23152 is ~350kDa.Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. 靶标信息Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM's C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

数据：

ATM Antibody (MA1-23152) in IHC (P) Immunohistochemical analysis of ATM in formalin-fixed, paraffin-embedded human kidney tissue using an ATM monoclonal antibody (Product # MA1-23152) at a dilution of 5 µg/mL followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. ATM Antibody (MA1-23152) in WBWestern blot analysis of ATM was performed by separating 30 µg of HeLa whole cell extract and nuclear extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATM Monoclonal Antibody (2C1) (Product # MA1-23152) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

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