Phospho-ATM (Ser1981) Monoclonal Antibody (10H11)

货号：MA1-2020

规格：200 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues S(1974

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：10H11

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:250(ICC);1:250(IF)；1:500 - 1:1000（WB）

产品详细信息MA1-2020 detects the phospho-ATM kinase in human and mouse samples.MA1-2020 has been successfully used in immunofluorescence, immunoprecipitation and Western blot procedures. By Western blot this antibody detects a ~370 kDa protein representing the phospho-ATM kinase in crude lysates from gamma irradiated HeLa cells. In immunofluorescence procedures, MA1-2020 recognizes the phospho-ATM kinase in irradiated human and mouse fibroblasts.The MA1-2020 immunogen is a phosphorylated synthetic peptide corresponding to the residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein. 靶标信息Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM's C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

数据：

Phospho-ATM (Ser1981) Antibody (MA1-2020) in IFImmunofluorescence analysis of Phospho-ATM pSer1981 was performed using 70% confluent log phase HeLa cells irradiated with UV for 4 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-ATM (Ser 1981) (10H11) Mouse Monoclonal Antibody (Product # MA1-2020) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the untreated cells. Panel f represents the no primary antibody control. The images were captured at 60X magnification. Phospho-ATM (Ser1981) Antibody (MA1-2020) in WBWestern blot analysis of phospho-ATM (pSer1981) was performed by loading 40 µg of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST (Product # 37525) for at least 1 hour. The membrane was probed with a phospho-ATM (pSer1981) monoclonal antibody (Product # MA1-2020) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

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