CD335 (NKp46) Monoclonal Antibody (9E2), PerCP-eFluor 710,

货号：46-3359-42

规格：100 Tests

市场价格：3654

产品类型：流式抗体

品牌：eBioscience

抗原：CD335 (NKp46)

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：9E2

荧光染料：PerCP-eFluor™ 710

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test(Flow)

产品详细信息Description: This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46. Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44. This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells. CD335 also interacts with pancreatic beta cells during the development of type Idiabetes.The 9E2 antibody has been reported to induce cytotoxicity.Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 9E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

数据

CD335 (NKp46) Antibody (46-3359-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) FITC (Product # 11-0569) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD335 (NKp46) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品

二抗荧光二抗免疫组化一抗标签抗体

技术参数

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-48871.htm