MST1/STK4 Polyclonal Antibody

货号：A300-465A

规格：100 µL

价格：6082

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Between 375 and 425

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	2-5 µg/mg lysate(IP);1:500-1:2,500(WB)

产品详细信息Positive Samples: HepG2, HeLa, A-549; Cellular Location: Nucleus, nucleolus 靶标信息This gene encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.

数据：

MST1/STK4 Antibody (A300-465A) in WB Detection of MST1 and MST2 by western blot and immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 µg), Ramos (R, 50 µg) and mouse NIH3T3 (M, 50 µg) cells. B) Whole cell lysate (1 mg/IP reaction; 1/4 of reaction loaded/gel) from HeLa cells. Antibodies: Affinity purified rabbit anti-MST1 antibody A300-465A used at 1 µg/ml for WB (A and B) and 3 µg/mg lysate for IP. MST1 and MST2 were simultaneously immunoprecipitated using MST1/2 antibodies A300-466A and A300-468A. MST2 was immunoprecipitated using MST2 antibody A300-467A. Detection: Chemiluminescence with exposure times of 30 (A) and 3 (B) seconds. A300-465A is specific for MST1 and A300-467A is specific for MST2 as determined by reciprocal IP/WB.

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