Human PCNA ELISA Kit ELISA试剂盒,Human PCNA ELISA Kit/人PCNA ELISA试剂盒

2024-10-24

Human PCNA ELISA Kit/人PCNA ELISA试剂盒

货号:EH361RB,EH361RBX5,EH361RBX10

规格:96 tests,5X96 tests,10X96 tests

价格:6641,29884,57271

产品类型:Platinum ELISA

品牌:Thermo Fisher

物种:人

宿主:其它

抗体亚型:其它

荧光染料:其它

灵敏度

2.46 ng/mL

检测范围2.5-600 ng/mL

样本类型/体积

血清 50 μL

血浆 50 μL

上清液 50 μL

操作时间

1 hr 20 min

测试时间

4 hr 45 min

Homogenous (no wash)

No

批间变异系数(CV)<12%
批内变异系数(CV)<10%

仪器设备

Colorimetric Microplate Reader 酶标仪

规格

5 x 96 Tests

组份

- 预包被96孔板

- 标准品

- 测定稀释浓缩液

- 生物素化检测抗体

- SAV-HRP

- 洗涤液

- 显色剂

- 终止液

- 胶板盖

储存温度

2-8℃

Protein namePCNA
Protein aliasesCyclin, DNA polymerase delta auxiliary protein, PCNA, Proliferating cell nuclear antigen

物种(Tested)

试剂盒类型

Sandwich ELISA Kit

标记(染料)

HRP

检测抗体偶联物Biotin
Gene aliasesATLD2, PCNA
Gene ID(Human) 5111
Gene symbolPCNA
UniProt ID(Human) P12004
Human PCNA quantitates human PCNA in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PCNA.人PCNA定量测定血清,血浆,上清液中的人PCNA。 该检测试剂盒将专门识别天然和重组人PCNA。Target information 靶标信息PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.PCNA(聚合酶δ辅助蛋白)对于DNA复制必不可少,并且参与DNA切除和错配修复途径。 PCNA与CDK抑制剂p21,结构特异性核酸内切酶Fen1和XPG以及DNA胞嘧啶5-甲基转移酶(MCMT)结合。 PCNA是具有增殖潜力并用于鉴定肿瘤组织的增殖状态(即与预后有关)的细胞的潜在有用的标志物。 PCNA是细胞周期早期G1期和S期细胞的标志物。 PCNA存在于细胞核中,是DNA聚合酶δ的辅因子,可作为同源三聚体并有助于增加DNA复制过程中前导链合成的过程。响应DNA损伤,PCNA被泛素化并参与RAD6依赖性DNA修复途径。已经为PCNA发现了两种编码相同蛋白质的转录物变体。 PCNA的假基因已在4号染色体和X染色体上描述。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。不用于诊断过程。未经明确授权不得转售。

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