AMPK alpha-1 Monoclonal Antibody (9Q34)

货号：AHO1332

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant fragment of human AMPK-alpha-1 express

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：9Q34

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:100(ICC);1:100(IF)；1:100(WB)

靶标信息Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

数据：

AMPK alpha-1 Antibody (AHO1332) in IF Immunofluorescence analysis of AMPK alpha-1 was performed using 70% confluent log phase LNCap cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with AMPK alpha-1 Monoclonal Antibody (9Q34)(Product # AHO1332) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. AMPK alpha-1 Antibody (AHO1332) in WB Western blot analysis was performed on whole cell extracts (20 µg lysate) of HeLa (lane 1), L6 (lane 2), K562 (lane 3), Jurkat (lane 4), MCF7 (lane 5), Hep G2 (lane 6), HT-29 (lane 7), HCT 116 (lane 8) and MDA-MB-231 (lane 9). The blots were probed with Anti-AMPK alpha Mouse Monoclonal Antibody (Product # AHO1332, 1:500 dilution) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 60 kDa band corresponding to AMPK alpha was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

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