PRMT4 Monoclonal Antibody (3H2)

货号：MA5-15796

规格：100 µL

价格：5764

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Purified recombinant fragment of human CARM1 expre

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：3H2

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	4 µL/10^6(ChIP);1/200 - 1/400(Flow);1:200-1:1000(ICC);1/200 - 1/1000(IF)；1/200 - 1/1000（IHC）;1/500 - 1/2000(WB)

产品详细信息MA5-15796 targets CARM1 in FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, Mouse and Rat samples.The MA5-15796 immunogen is purified recombinant fragment of human CARM1 expressed in E. Coli.MA5-15796 detects CARM1 which has a predicted molecular weight of approximately 65kDa. 靶标信息Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

数据：

PRMT4 Antibody (MA5-15796) in IFImmunofluorescence analysis of PRMT4 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRMT4 Monoclonal Antibody (3H2) (Product # MA5-15796) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. PRMT4 Antibody (MA5-15796) in WBWestern blot analysis of CARM1 was performed by loading 20 µg from p53-/- Mouse Embryonic Fibroblasts (MEFs) treated with a control shRNA (shNT) or four different shRNA's (sh#1-sh#4) knocking down CARM1 protein per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in PBST for 1 hour. The membrane was probed with a CARM1 monoclonal antibody (Product # MA5-15796) at a dilution of 1:1000 at 4°C overnight, washed in PBST, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:1000 for 1 hour at room temperature. As a loading control, the same lysates were probed with an antibody against alpha-Tubulin. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

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