NK1.1 Monoclonal Antibody (PK136), Biotin, eBioscience™

货号：13-5941-82,13-5941-81, 13-5941-85

规格：100 µg,50µg,500 µg

价格：1518,822,2534

产品类型：流式抗体

品牌：eBioscience

抗原：NK1.1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：PK136

荧光染料：Biotin

类型:	一抗	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™（#13-4724-85）
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: PK136 has been reported for use in flow cytometric analysis.Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息NK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.仅用于科研。不用于诊断过程。

数据

NK1.1 Antibody (13-5941-82) in FlowStaining of C57BL/6 mouse splenocytes with Anti-Mouse NK-1.1 PE and Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82). Total viable cells were used for analysis.

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参考文献：

1. Proceedings of the National Academy of Sciences of the United States of AmericaPre/pro-B cells generate macrophage populations during homeostasis and inflammation."13-5941 was used in Flow cytometry/Cell sorting to study the developmental plasticity of immature B cells and their role as the source of tissue resident macrophages."AuthorsAudzevich T,Bashford-Rogers R,Mabbott NA,Frampton D,Freeman TC,Potocnik A,Kellam P,Gilroy DWYear2017SpeciesMouse 2. CellA Viral Immunoevasin Controls Innate Immunity by Targeting the Prototypical Natural Killer Cell Receptor Family."13-5941 was used in Flow cytometry/Cell sorting to identify a viral ligand for the inhibitory and activating NKR-P1 receptors."AuthorsAguilar OA,Berry R,Rahim MMA,Reichel JJ,Popović B,Tanaka M,Fu Z,Balaji GR,Lau TNH,Tu MM,Kirkham CL,Mahmoud AB,Mesci A,Krmpotić A,Allan DSJ,Makrigiannis AP,Jonjić S,Rossjohn J,Carlyle JRYear2017SpeciesMouse 3. European journal of immunologymTORC2 regulates multiple aspects of NKT-cell development and function."13-5941 was used in Flow cytometry/Cell sorting to define the molecular pathway and role of Rictor in regulating invariant NKT-cell fate."AuthorsSklarz T,Guan P,Gohil M,Cotton RM,Ge MQ,Haczku A,Das R,Jordan MS 4. Frontiers in neurologyIL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury."Published figure using NK1.1 monoclonal antibody (Product # 13-5941-82) in Flow5. Cytometry"Nature communicationsSynergized regulation of NK cell education by NKG2A and specific Ly49 family members."Published figure using NK1.1 monoclonal antibody (Product # 13-5941-82) in Flow Cytometry"

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