SETD7 Polyclonal Antibody

货号：PA5-81017

规格：100 µL

价格：1698

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Recombinant Human SETD7/SET7/9 protein

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:5000-1:10000(ELISA);1-4 µL/mg of lysate(IP);1:500-1:2000(WB)

产品详细信息This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).This antibody has specificity for Human SETD7/SET7/9. 靶标信息Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. [UniProt]

数据：

SETD7 Antibody (PA5-81017) in WBWestern blot analysis of SETD7 in Lane A: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a SETD7 Polyclonal Antibody (Product # PA5-81017) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:48 kDa. Observed band size:53 kDa. SETD7 Antibody (PA5-81017) in IP SETD7 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, 2 µL of SETD7 Polyclonal Antibody and 15 µL of 50% Protein G agarose. Primary Antibody: SETD7 Polyclonal Antibody (Product # PA5-81017), at 1:200 dilution. Secondary Antibody: Clean-Blot IP Detection Reagent (HRP) at 1:500 dilution. Immunoprecipitation was performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 52 kDa.

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