SETDB1 Monoclonal Antibody (5H6A12)

货号：MA5-15722

规格：100 µL

价格：5463

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Purified recombinant fragment of human SETDB1 expr

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：5H6A12

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:200-1:1000(ICC);1/200 - 1/1000(IF);1/500 - 1/2000(WB)

产品详细信息MA5-15722 targets SETDB1 in IF and WB applications and shows reactivity with Human, mouse, and Non-human primate samples.The MA5-15722 immunogen is purified recombinant fragment of human SETDB1 expressed in E. Coli.MA5-15722 detects SETDB1 which has a predicted molecular weight of approximately 180kDa. 靶标信息This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

数据：

SETDB1 Antibody (MA5-15722) in IFImmunofluorescence analysis of LOVO cells using SETDB1 monoclonal antibody (Product # MA5-15722) (Green). Red: actin filaments have been labeled with phalloidin. SETDB1 Antibody (MA5-15722) in WB Western blot was performed using Anti-SETDB1 Monoclonal Antibody (5H6A12) (Product # MA5-15722) and a 180 kDa band corresponding to SETDB1 was observed in all cell and tissue lysates tested. Modified whole cell lysates (1% SDS) (30 µg lysate) of SW480 (Lane 1), MCF7 (Lane 2), T-47D (Lane 3), IMR-32 (Lane 4), and tissue lysates (30 µg lysate) of Mouse Testis (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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