SETDB1 Polyclonal Antibody

货号：PA5-79982

规格：100 µg

价格：2628

产品类型：标签抗体

品牌：Thermo Fisher

抗原：E. coli-derived human KMT1E/SETDB1 recombinant pro

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	500 µg/mL	用法：	0.1-0.5 µg/mL(WB)

产品详细信息Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL. 靶标信息This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

数据：

SETDB1 Antibody (PA5-79982) in IHC (P)Immunohistochemistry analysis of SETDB1 on paraffin-embedded human rectal cancer tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with SETDB1 polyclonal antibody (Product# PA5-79982) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method. SETDB1 Antibody (PA5-79982) in WB Western blot analysis of SETDB1 in Lane 1: human placenta tissue lysate, Lane 2: rat brain tissue lysate, Lane 3: rat ovary tissue lysate, Lane 4: mouse brain tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with SETDB1 polyclonal antibody (Product # PA5-79982) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

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