Mouse anti Human CD16:Amethyst Orange 伯乐CD16抗体 MCA1193AMO-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD16:Amethyst Orange 伯乐CD16抗体 MCA1193AMO

2024-10-28

Mouse anti Human CD16:Amethyst Orange

货号：MCA1193AMO

规格：0.1 mg

市场价格：2525

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD16

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：LNK16

荧光染料：Amethyst Orange

产品介绍
Mouse anti Human CD16 antibody, clone LNK16recognizes CD16, a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes.CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Mouse anti Human CD16 antibody, clone LNK16 has been reported to block the binding of IgG to the CD16 molecule (Tamm and Schmidt 1996).
产品详情
Target Species	Human
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by ion exchange chromatography
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin
Immunogen	Normal human peripheral blood granulocytes.
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
Max Ex/Em	405/540
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 10⁶cells or 100ul human whole blood.
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数据
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD56 (MCA2693A488) and Amethyst Orange conjugated Mouse IgG1 isotype control (MCA928AMO). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD56 (MCA2693A700) and Amethyst Orange conjugated Mouse anti Human CD16 (MCA1193AMO). All experiments performed on human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
文献
1. Tamm, A. & Schmidt, R.E. (1996) The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding.J Immunol. 157 (4): 1576-81.2. Fleit, H.B.et al.(1982) Human neutrophil Fc gamma receptor distribution and structure.Proc Natl Acad Sci U S A. 79 (10): 3275-9.3. van de Winkel, J.G. & Capel, P.J. (1993) Human IgG Fc receptor heterogeneity: molecular aspects and clinical implications.Immunol Today. 14 (5): 215-21.4. Pillay, J.et al.(2012) A subset of neutrophils in human systemic inflammation inhibits T cell responses through Mac-1.J Clin Invest. 122: 327-36.5. Morris, D.L.et al.(2010) Evidence for both copy number and allelic (NA1/NA2) risk at the FCGR3B locus in systemic lupus erythematosus.Eur J Hum Genet. 18 (9): 1027-31.6. Kamp, V.M.et al.(2012) Human suppressive neutrophils CD16bright/CD62Ldim exhibit decreased adhesion.J Leukoc Biol. 92 (5): 1011-20.7. Hanna, J.et al.(2003) CXCL12 expression by invasive trophoblasts induces the specific migration of CD16- human natural killer cells.Blood. 102:1569-77.8. Moalli, F.et al.(2010) Role of complement and Fc{gamma} receptors in the protective activity of the long pentraxin PTX3 against Aspergillus fumigatus.Blood. 116: 5170-80.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Human CD16:Amethyst Orange 伯乐CD16抗体 MCA1193AMO

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