EHMT1 Polyclonal Antibody

货号：A301-642A

规格：100 µL

价格：6082

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Between 25 and 75

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.20 mg/mL	用法：	1:100-1:500(IHC);2-5 µg/mg lysate(IP);1:2,000-1:10,000(WB)

产品详细信息The recommended shelf life for this product is 1 year from date of receipt.Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. 靶标信息EHMT1, also known as EUHMTASE1, is a histone methyltransferase. This protein methylates 'Lys-9' of histone H3 in vitro. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. EHMT1 is Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in the G0/G1 transition of the cell cycle.

数据：

EHMT1 Antibody (A301-642A) in IHCDetection of human EHMT1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-EHMT1 (Cat. No. A301-642A) used at a dilution of 1:200 (1µg/ml). Detection: DAB. EHMT1 Antibody (A301-642A) in WBDetection of human EHMT1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EHMT1 antibody A301-642A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. EHMT1 was also immunoprecipitated by rabbit anti-EHMT1 antibody A301-643A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).

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