PIM1 Recombinant Polyclonal Antibody (19HCLC)

货号：710504

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Peptide corresponding to amino acids 115–130 of human PIM-1

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：19HCLC

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:50(IHC(P));1 µg/mL(WB)

产品详细信息This antibody is predicted to react with bovine, canine, equine, feline, non-human primate, mouse, rabbit, ovine and porcine based on sequence homology.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. 靶标信息PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.

数据：

PIM1 Antibody (710504) in IHC (P)Immunohistochemistry analysis of Pim-1 showing staining in the cytoplasm of paraffin-embedded mouse testis tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Pim-1 Antibody (19HCLC) Recombinant Rabbit Polyclonal (Product # 710504) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. PIM1 Antibody (710504) in WB Western blot analysis of Pim-1 in whole cell extracts of Raji and Jurkat cells (lanes 1-2) using a Pim-1 Recombinant Rabbit Polyclonal Antibody (Product # 710504) at a dilution of 1 µg/mL. Detection was performed using an HRP-conjugated Goat anti-Rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~34kDa.

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