SRC1 Polyclonal Antibody

货号：PA1-840

规格：100 µg

价格：5468

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues S(8) S

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1-2 µg/mL(ICC);1-2 µg/mL(IF) ;1 µg/mL(IHC(F));1 µg/mL(WB)

产品详细信息PA1-840 detects steroid receptor coactivator-1 (SRC-1) from human, mouse, and japaneese quail tissues and cells.PA1-840 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~165 kDa protein representing SRC-1 and a smaller, unknown protein at ~40 kDa from HeLa cell lysate. Immunocytochemical staining of SRC-1 in HeLa cells with PA1-840 results in nuclear staining.The PA1-840 immunogen is a synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1. This immunizing peptide (Cat. # PEP-044) is available for use in neutralization and control experiments. 靶标信息Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

数据：

SRC1 Antibody (PA1-840) in IF Immunofluorescence analysis of SRC1 was done on 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with SRC1 Rabbit Polyclonal Antibody (Product # PA1-840) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification. SRC1 Antibody (PA1-840) in IHCImmunohistochemical staining of SRC-1 in the Dentate Gyrus region of the rat brain using Product # PA1-840. SRC1 Antibody (PA1-840) The Journal of neuroscience : the official journal of the Society for Neuroscience 2005 - Published figure using SRC1 polyclonal antibody (Product # PA1-840)

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