SRC1 Monoclonal Antibody (1135/H4)

货号：MA1-840

规格：100 µL

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to residues 477-94

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1135/H4

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	5 µL / 10^6 cells(ChIP);1 µg / 10^6 cells(Flow);1:200(IHC(P));1:500-1:5000(WB)

产品详细信息MA1-840 detects steroid receptor coactivator-1 (SRC-1) from mouse and primate tissues and cells as well as recombinant human SRC-1 protein.MA1-840 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot MA1-840 detects an ~165 kDa protein representing SRC-1 in COS cell extract. Also by Western blot, MA1-840 detects an unidentified protein at ~30-40 kDa. This antibody does not react with NIH-3T3 cells in Western blot.The MA1-840 immunogen is a synthetic peptide corresponding to residues 477-947 of human SRC-1. The epitope for MA1-840 has been mapped to the extreme C-terminal end of this fragment. 靶标信息Clone 1135 recognizes SRC-1, steroid-receptor coactivator-1. SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

数据：

SRC1 Antibody (MA1-840) in WBWestern blot analysis of SRC1 was performed by loading 25 µg of LNCaP (lane 1), PC-3 (lane 2) and NIH-3T3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a SRC1 monoclonal antibody (Product # MA1-840) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~152 and ~157 kDa.

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