Phospho-JAK2 (Tyr1007, Tyr1008) Polyclonal Antibody

货号：44-426G

规格：100 µL

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：The antiserum was produced against a chemically sy

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:1000（WB）

靶标信息Janus Activating Kinase 2 (JAK2) is a non-rececptor tyrosine kinase involved in various processes such as cell growth, development, differentiation, and histone modifications. In response to a varitety of cytokine or related factors (e.g., interferon, interleukins), JAKs are activated via phosphorylation at 2 adjacent tyrosine residues. The activation of JAKs can lead to the phosphorylation of STAT (signal transducers and activatiors of transcription) proteins, which dimerize and translocate to the nucleus. Once translocated to the nucleus, the STAT proteins can modify transcription of numerous genes, including interferon-stimulated genes. JAK2 is required for the IFN gamma-receptor complex initiation and JAK1 functions as an amplifier. Some studies have suggested that the role of JAK2 might be performed by Tyk2 and JAK3, if they were positioned correctly within the IFN gamma-receptor complex. Mutations affecting this gene can cause Budd-Chiari syndrome, Polycythemia vera, Thrombocythemia 3, myelofibrosis, or Acute myelogenous leukemia.

数据：

Phospho-JAK2 (Tyr1007, Tyr1008) Antibody (44-426G) in WB Western blot was performed using Phospho-JAK2 (Tyr1007, Tyr1008) (Product # 44-426G) and ~130 kDa band corresponding to JAK2 (Tyr1007, Tyr1008) was observed. Whole cell extracts of 3T3-L1 lysate (Lane 1), 3T3-L1 treated with 50 ng/mL of Leukemia Inhibitory Factor (LIF) for 15 min (lane 2), Antibody treated with non-phospho peptide (Lane 3) and Antibody treated with phospho peptide (Lane 4) were electrophoresed using Novex® NuPAGE® Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected with Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Phospho-JAK2 (Tyr1007, Tyr1008) Antibody (44-426G)Molecular and cellular biology 2015 - Published figure using Phospho-JAK2 (Tyr1007, Tyr1008) polyclonal antibody (Product # 44-426G)

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