Rabbit anti Uracil-DNA glycolase 1 antibody AHP1201 bio-rad,Rabbit anti Uracil-DNA glycolase 1 antibody

2024-10-22

Rabbit anti Uracil-DNA glycolase 1 antibody

货号:AHP1201

规格:0.1 mg

价格:5287

产品类型:免疫组化一抗

品牌:Bio-Rad

物种:人

宿主:山羊

抗体亚型:IgG

荧光染料:Purified

产品介绍
Rabbit anti Uracil-DNA glycolase 1 antibodyrecognizes uracil-DNA glycosylase 1 (UNG1), a member of the uracil-DNA glycosylase and cyclin family. Two isoforms of UNG exist, isoform 1 is expressed in the mitochondria of in skeletal muscle, heart and testicles while isoform 2 (UNG2) is more abundant in proliferating tissues and expressed in the nucleus.Uracil residues can be misincorporated into DNA by DNA polymerase or by deamination of cytosine. UNG1 is a major enzyme in base excision repair, excising the misincorporated uracil residues in a process crucial to increasing immunoglobulin diversity. In association with activation-induced cytosine deaminase, UNG is also essential to the generation of strand breaks that initiate class switch recombination.UNG is also involved in the innate immune response against retroviral infections, HIV-1 accessory protein Vpr induces the rapid degradation of UNG2.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
Buffer SolutionPhosphate buffered saline
Antiserum PreparationAntisera to human uracil-DNA glycosylase 1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen14 amino acid peptide sequence near the amino terminus of human UNG1.
Approx. Protein ConcentrationsIgG concentration 1.0mg/ml
RegulatoryFor research purposes only
GuaranteeGuaranteed until date of expiry. Please see product label.
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining alquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting0.51.0ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
二抗
DescriptionProduct CodeApplicationsPack Size
Goat anti Rabbit IgG (Fc):BiotinSTAR121BE,WB1mg
Goat anti Rabbit IgG (H/L):HRPSTAR124PC,E,WB1mg
文献
1.Hagen, L.et al. (2007) Genomic uracil and human disease.Exp Cell Res. 312:2666-2672.2.Krokan, H.E.et al.(2001) Properties and functions of human uracil-DNA glycosylase from the UNG gene.Prog Nucleic Acid Res Mol Biol. 68: 365-86.3.Akbari, M.et al.(2007) Different organization of base excision repair of uracil in DNA in nuclei and mitochondria and selective upregulation of mitochondrial uracil-DNA glycosylase after oxidative stress.Neuroscience. 145 (4): 1201-12.

技术参数

产品应用 wb

产品类型 Polyclonal Antibody

推荐用于 wb

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