PPP4C Monoclonal Antibody (OTI2E4)

货号：MA5-27269

规格：100 µL

价格：5956

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Full length human recombinant protein of PPP4C pro

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：OTI2E4

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1:2000(IHC(P));1:500-1:2000(WB)

产品详细信息The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

数据：

PPP4C Antibody (MA5-27269) in IHC (P)Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a PPP4C monoclonal antibody (Product # MA5-27269) at a dilution of 1:2000 PPP4C Antibody (MA5-27269) in WBWestern blot analysis of PPP4C in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PPP4C (Product # MA5-27269) monoclonal antibody.

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