HDAC2 Polyclonal Antibody

货号：51-5100

规格：100 µg

价格：4809

产品类型：标签抗体

品牌：Thermo Fisher

抗原：A synthetic 11 amino acid peptide derived from the

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	0.25 mg/mL	用法：	0.1-1.0 µg/mL(ELISA) ;5 µg/mL(ICC);5 µg/mL(IF);1:20(IHC(P));5 µg(IP);1-3 µg/mL(WB)

靶标信息HDAC2 belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

数据：

HDAC2 Antibody (51-5100) in IFImmunofluorescence analysis of HDAC2 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HDAC2 Polyclonal Antibody (Product # 51-5100) at 5 µg/ml in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. HDAC2 Antibody (51-5100) in IHC (P)Immunohistochemistry analysis of HDAC2 showing staining in the cytoplasm and nucleus of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- HDAC2 Polyclonal Antibody (Product # 51-5100) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting. HDAC2 Antibody (51-5100)Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with HDAC2 siRNA and reduction of signal was observed in Western Blot using HDAC2 Polyclonal Antibody (Product # 51-5100).Knockdown validation info.

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技术参数

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