JARID1B Polyclonal Antibody

货号：A301-813A

规格：100 µL

价格：6082

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Between 1494 and 1544

物种：人/小鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	2-10 µg/mg lysate(IP);1:500-1:2,000(IHC);1:2,000-1:10,000(WB)

产品详细信息The recommended shelf life for this product is 1 year from date of receipt.Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.靶标信息KDM5B is a histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. KDM5B demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. It acts as a transcriptional corepressor for FOXG1B and PAX9. It favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, KDM5B may act as a tumor suppressor for melanoma.

数据：

JARID1B Antibody (A301-813A) in IHCDetection of human Jarid1B by immunohistochemistry.Sample:FFPE section of human prostate carcinoma.Antibody:Affinity purified rabbit anti-Jarid1B (Cat. No. A301-813A Lot2) used at a dilution of 1:500 (2µg/ml).Detection:DAB. JARID1B Antibody (A301-813A) in WBDetection of human Jarid1B by western blot of immunoprecipitates.Samples:Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.Antibodies:Affinity purified rabbit anti-Jarid1B antibody A301-813A (lot A301-813A-2) used for IP at 6 µg per reaction. Jarid1B was also immunoprecipitated by a previous lot of this antibody (lot A301-813A-1) and rabbit anti-Jarid1B antibody BL4377 For blotting immunoprecipitated Jarid1B, A301-813A was used at 1 µg/ml.Detection:Chemiluminescence with an exposure time of 30 seconds.

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技术参数

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