SIRT6 Polyclonal Antibody

货号：PA5-17215

规格：100 µL

价格：4880

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Synthetic peptide corresponding to the carboxy ter

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：		用法：	1:100(ICC)；1:100（IF）；1:50（IP）；1:1000（WB）

产品详细信息It is not recommended to aliquot this antibody.This antibody is not cross-reactive with other sirtuin proteins.靶标信息Sirtuins regulate epigenetic gene silencing. SIRT6 (Mono-ADP-ribosyltransferase sirtuin-6), a mouse Sir2 homolog, is a chromatin-associated nuclear ADP-ribosyltransferase protein. It appears to be involved in DNA repair and may also play a role in human aging.

数据：

SIRT6 Antibody (PA5-17215) in IFImmunofluorescence analysis of SIRT6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SIRT6 Polyclonal Antibody (Product # PA5-17215) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. SIRT6 Antibody (PA5-17215) in WBWestern blot analysis of SirT6 in Hela and Jurkat cells using SirT6 polyclonal antibody (Product # PA5-17215).

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