53BP1 Polyclonal Antibody

货号： PA1-16565

规格：100 µL

价格：5548

产品类型：标签抗体

品牌：Thermo Fisher

抗原：The epitope recognized by this antibody maps to a

物种：其它

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	1.5 µg/mL（Flow）;1:1000-1:5000(ICC);1:1000-1:5000(IF);1:1000-1:5000(IHC(P));1:5000-1:25,000(WB)

产品详细信息Human has been tested in WB, IHC-P and ICC/IF, mouse and goat have only been tested in ICC/IF.Suggested positive control: U205, 293T, MO59K cell lysates and MEF lysates .靶标信息53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a putative substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to the presumptive sites of damage, specifically, double-strand breaks. 53BP1 may have a role in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and participates in DNA repair by maintaining genomic stability.

数据：

53BP1 Antibody (PA1-16565) in IF53BP1 foci in proliferating MO59K cells; both normal and exposed to 10 Gy of IR and double-color immunofluorescence stained after 2 hours. Images captured in a Kodak digital image system on a Leica fluorescent microscope. 53BP1 Antibody (PA1-16565) in WBWestern blot was performed using Anti-53BP1 Polyclonal Antibody (Product # PA1-16565) and a ~270kDa band corresponding to TP53-binding protein 1 was observed across cell lines tested . Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4), U-2 OS (Lane 5), K-562 (Lane 6), U-87 MG (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:10000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).A streak like pattern was observed in the positive cell lines which is expected of 53BP1 target.

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